ITGA4 抗体 (Biotin)
Quick Overview for ITGA4 抗体 (Biotin) (ABIN114224)
抗原
See all ITGA4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This monoclonal antibody reacts with a4 integrin, which helps to mediate cell-cell and cell-matrix interactions.
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纯化方法
- Protein G Chromatography
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免疫原
- Peyers Patch HEV binding lymphoma line (TK1) Donor: Fisher Spleen Fusion Partner: P3x63Ag8.653
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亚型
- IgG2b
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anti-Integrin alpha 4 (ITGA4) (Internal Region) antibody
ITGA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 551-650) antibodyITGA4 适用: 人, 小鼠, 兔 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 833-977) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7E4C5 unconjugated
anti-Integrin alpha 4 (ITGA4) (C-Term) antibodyITGA4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 376-558) antibodyITGA4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, EIA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) (C-Term) antibodyITGA4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro), Func 宿主: 大鼠 Monoclonal DATK32 unconjugated
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应用备注
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Flow cytometry. Immunoprecipitation. Immunohistochemistry. (1,2,3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2bCell Source: Percentage of cells stained above control: TK1 Cells: 97. 9%Thymus: 80. 0%Spleen: 85. 1%Bone Marrow: 72. 4%
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ITGA4 (Integrin alpha 4 (ITGA4))
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别名
- CD49d / ITGA4
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背景
- Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
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基因ID
- 16401
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NCBI登录号
- NP_034706
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UniProt
- Q00651
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途径
- Integrin Complex
抗原
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